Purification and properties of a yeast nucleotide pyrophosphatase.

Purification and properties of yeast pyrophosphatase.

Purification of yeast inorganic pyrophosphatase.

An enzyme which hydrolyzes inorganic pyrophosphate (PP) to orthophosphate (ortho P) was demonstrated by Lohmann (1) in muscle. Bauer (2) found that autolysates of slowly dried bottom yeast were a rich source of inorganic pyrophosphatase and were free of adenosinetriphosphatase (ATPase) activity. An important contribution was made by Bailey and Webb (3) who accomplished an extensive purification...

The purification and properties of detergent-solubilized rat liver nucleotide pyrophosphatase.

Nucleotide pyrophosphatase was solubilized from the microsomal fraction of rat liver with Triton X-100 and was purified by ion exchange chromatography on DEAE-cellulose, gel filtration through Sepharose 4B and preparative disc electrophoresis in the presence of 0.1% sodium dodecyl sulfate. This procedure resulted in a final purification of 176-fold. Maintenance of enzymatic activity through the...

Purification of Maize Nucleotide Pyrophosphatase/Phosphodiesterase Casts Doubt on the Existence of Zeatin Cis–Trans Isomerase in Plants

Almost 25 years ago, an enzyme named zeatin cis-trans isomerase from common bean has been described by Bassil et al. (1993). The partially purified enzyme required an external addition of FAD and dithiothreitol for the conversion of cis-zeatin to its trans- isomer that occurred only under light. Although an existence of this important enzyme involved in the metabolism of plant hormones cytokini...

Effects of yeast nucleotide on growth performance, serum immune index and muscle composition of Ancherythroculter nigrocauda Yih & Wu

A 50-day feeding trial was conducted to evaluate the effects of yeast nucleotide in diets on growth performance, serum immune indices and muscle composition of Ancherythroculter nigrocauda (mean initial body weight, 23.30±0.59 g). Seven isonitrogenous (approximately 42.76% crude protein) and isoenergetic (17.43 KJ g−1 gross energy) experimental diets with varying levels of yeast nucleotide (0[c...